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Ammonium sulphate
Smith and K
Component g/l Soya Peptone 16 Yeast extract 10 NaCl 5 Final pH 7
Pepsin will cut the peptide chain anywhere there is a phenylalanine or leucine bond
These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants
Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Copper Size:1000g Ammonium sulphateCupric sulphate, CuSO4. 5H2O, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of copper or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at
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