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By omitting Folic acid and Riboflavin from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium
LBM-Broth Low Salt
10 g/L Glycerol 315
0003% Ca (Calcium) <0
Concentrations of Cysteine
LBM-Agar Miller Size:1000g By omitting Folic acid andLBM Agar Low Salt In LBM media Mg2 is added to enhance absorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Agar 15 Final pH 7. 0 0. 2 at 25C Suspend 41 gram powdered medium in 1 litre distilled water Store dry at room temperature