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ACTN3 control DNA – WT (wild type) (1 tubes á 100 µl
Therefore it is necessary to find the optimal reference gene for your conditions and to validate that this gene is indeed nonregulated
• TATAA SYBR GrandMaster Mix LowROX (V = 2 · 1250 µl
Features High sensitivity - optimized for two-step quantitative RT-PCR Broad dynamic range Unbiased cDNA synthesis - complete 5’ to 3’ RNA sequence representation Easy reaction assembly
Reference gene selection kit
TATAA Interplate Calibrator IPC kits:TATAA Interplate Calibrator 250 rxn SYBR® ACTN3 control DNA – WTVariation Compensation The TATAA Interplate Calibrator (IPC) makes it possible for you to compensate for variations between qPCR runs. For practical reasons many qPCR studies involve the use of samples that are processed in more than a single batch or in which the sample set is extended over time. Even over a short time period, variation between qPCR processing runs is observed due to different baseline subtractions and threshold settings. When to use